The impact of intermittent and continuous training on the levels of CIDE and Perilipin-1 proteins and their effect on the size of lipid droplets in the visceral adipose tissue of obese male rats

Yan  Bai; Xiao  Yang; Jian Li

doi:10.21134/eurjhm.2024.52.4

Authors

Yan Bai The School of Humanities and Social Sciences, Guangzhou Civil Aviation College
Xiao Yang The School of Humanities and Social Sciences, Guangzhou Civil Aviation College
Jian Li Asset Management Office, Guangzhou Sport University, Guangzhou 510500, Guangdong, China

DOI:

https://doi.org/10.21134/eurjhm.2024.52.4

Keywords:

High intensity interval training, CIDE, Lipid droplets, Moderate intensity continuous training, Prilipin-1

Abstract

Intense interval training and moderate-intensity continuous exercise produce lipid droplets that change size and impact visceral adipose tissue. 50 male Wistar rats were divided into 5 groups, each consisting of 8 rats, in order to accomplish this objective. Regarding dietary intake, 2 clusters of 32 rats were subjected to a normal or elevated fat diet over 10 weeks. Post the induction of obesity, 16 animals were euthanized, with an equal number originating from both the high-fat and normal diet cohorts. The ramifications of a high-fat diet were examined through the utilization of samples. The remaining 24 rats were randomly allocated to 3 groups: a sedentary high-fat diet control group, a high-intensity interval training (HIIT) protocol group, and a moderate-intensity continuous training (MICT) protocol group. The 12-week training program had 5 sessions per week. Western blot measurement of perilipin-1, CIDE, and Oil-Red proteins assessed lipid droplet size. Research indicates that HIIT and MICT training significantly decreased CIDEc protein levels (p<0.05) but not CIDEa. CIDEc protein upregulation and perilipin-1 downregulation cause obesity in high-fat diets. HIIT and MICT training reduce fat droplet size and CIDEc protein production. Enhancing perilipin-1, which breaks down fats, may reduce obesity by lowering lipid droplets and weight.

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