The impact of intermittent and continuous training on the levels of CIDE and Perilipin-1 proteins and their effect on the size of lipid droplets in the visceral adipose tissue of obese male rats

Authors

  • Shaghayegh Radmehr Cheeloo College of Medicine, Shandong university, Jinan, China
  • Fatemeh Dehghani Pediatrics Department of Nursing, Iranshahr University Medical Sciences, Iranshahr, Iran
  • Yan Bai The School of Humanities and Social Sciences, Guangzhou Civil Aviation College
  • Xiao Yang The School of Humanities and Social Sciences, Guangzhou Civil Aviation College
  • Jian Li Asset Management Office, Guangzhou Sport University, Guangzhou 510500, Guangdong, China

DOI:

https://doi.org/10.21134/eurjhm.2024.52.4

Keywords:

High intensity interval training, CIDE, Lipid droplets, Moderate intensity continuous training, Prilipin-1

Abstract

Intense interval training and moderate-intensity continuous exercise produce lipid droplets that change size and impact visceral adipose tissue. 50 male Wistar rats were divided into 5 groups, each consisting of 8 rats, in order to accomplish this objective. Regarding dietary intake, 2 clusters of 32 rats were subjected to a normal or elevated fat diet over 10 weeks. Post the induction of obesity, 16 animals were euthanized, with an equal number originating from both the high-fat and normal diet cohorts. The ramifications of a high-fat diet were examined through the utilization of samples. The remaining 24 rats were randomly allocated to 3 groups: a sedentary high-fat diet control group, a high-intensity interval training (HIIT) protocol group, and a moderate-intensity continuous training (MICT) protocol group. The 12-week training program had 5 sessions per week. Western blot measurement of perilipin-1, CIDE, and Oil-Red proteins assessed lipid droplet size. Research indicates that HIIT and MICT training significantly decreased CIDEc protein levels (p<0.05) but not CIDEa. CIDEc protein upregulation and perilipin-1 downregulation cause obesity in high-fat diets. HIIT and MICT training reduce fat droplet size and CIDEc protein production. Enhancing perilipin-1, which breaks down fats, may reduce obesity by lowering lipid droplets and weight.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Ahmadian, M., Duncan, R. E., Jaworski, K., Sarkadi-Nagy, E., & Sook Sul, H. (2007). Triacylglycerol metabolism in adipose tissue. Future lipidology, 2(2), 229-237.

Barneda, D., & Christian, M. (2017). Lipid droplet growth: regulation of a dynamic organelle. Current Opinion in Cell Biology, 47, 9-15.

Boschi, F., Rizzatti, V., Zamboni, M., & Sbarbati, A. (2015). Models of lipid droplets growth and fission in adipocyte cells. Experimental Cell Research, 336(2), 253-262.

Bouchez, I., Pouteaux, M., Canonge, M., Genet, M. l., Chardot, T., Guillot, A., & Froissard, M. (2015). Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase. Biology open, 4(7), 764-775.

Brasaemle, D. L., & Wolins, N. E. (2012). Packaging of fat: an evolving model of lipid droplet assembly and expansion. Journal of Biological Chemistry, 287(4), 2273-2279.

De Farias, J., Bom, K., Tromm, C., Luciano, T., Marques, S., Tuon, T., . . . Pinho, R. (2013). Effect of physical training on the adipose tissue of diet-induced obesity mice: interaction between reactive oxygen species and lipolysis. Hormone and metabolic research, 45(03), 190-196.

Gao, G., Chen, F.-J., Zhou, L., Su, L., Xu, D., Xu, L., & Li, P. (2017). Control of lipid droplet fusion and growth by CIDE family proteins. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1862(10), 1197-1204.

Gong, J., Sun, Z., Wu, L., Xu, W., Schieber, N., Xu, D., . . . Li, P. (2011). Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contact sites. Journal of Cell Biology, 195(6), 953-963.

Hafstad, A. D., Boardman, N. T., Lund, J., Hagve, M., Khalid, A. M., Wisløff, U., . . . Aasum, E. (2011). High intensity interval training alters substrate utilization and reduces oxygen consumption in the heart. Journal of Applied Physiology, 111(5), 1235-1241.

Hafstad, A. D., Lund, J., Hadler-Olsen, E., Höper, A. C., Larsen, T. S., & Aasum, E. (2013). High-and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity. Diabetes, 62(7), 2287-2294.

Kimmel, A. R., & Sztalryd, C. (2016). The perilipins: major cytosolic lipid droplet–associated proteins and their roles in cellular lipid storage, mobilization, and systemic homeostasis. Annual review of nutrition, 36, 471-509.

Konige, M., Wang, H., & Sztalryd, C. (2014). Role of adipose specific lipid droplet proteins in maintaining whole body energy homeostasis. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1842(3), 393-401.

Lafontan, M., & Langin, D. (2009). Lipolysis and lipid mobilization in human adipose tissue. Progress in lipid research, 48(5), 275-297.

Lam, Y., Mitchell, A. J., Holmes, A. J., Denyer, G., Gummesson, A., Caterson, I., . . . Storlien, L. (2011). Role of the gut in visceral fat inflammation and metabolic disorders.

Marcelin, G., & Chua Jr, S. (2010). Contributions of adipocyte lipid metabolism to body fat content and implications for the treatment of obesity. Current opinion in pharmacology, 10(5), 588-593.

Marcinkiewicz, A., Gauthier, D., Garcia, A., & Brasaemle, D. L. (2006). The phosphorylation of serine 492 of perilipin a directs lipid droplet fragmentation and dispersion. Journal of Biological Chemistry, 281(17), 11901-11909.

Murphy, S., Martin, S., & Parton, R. G. (2009). Lipid droplet-organelle interactions; sharing the fats. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1791(6), 441-447.

Murphy, S., Martin, S., & Parton, R. G. (2010). Quantitative analysis of lipid droplet fusion: inefficient steady state fusion but rapid stimulation by chemical fusogens. PloS one, 5(12), e15030.

Oliveros, E., Somers, V. K., Sochor, O., Goel, K., & Lopez-Jimenez, F. (2014). The concept of normal weight obesity. Progress in cardiovascular diseases, 56(4), 426-433.

Sahu-Osen, A., Montero-Moran, G., Schittmayer, M., Fritz, K., Dinh, A., Chang, Y.-F., . . . Russell, D. (2015). CGI-58/ABHD5 is phosphorylated on Ser239 by protein kinase A: control of subcellular localization [S]. Journal of lipid research, 56(1), 109-121.

Schneider, M. R., Zhang, S., & Li, P. (2016). Lipid droplets and associated proteins in the skin: basic research and clinical perspectives. Archives of dermatological research, 308, 1-6.

Skinner, J. R., Harris, L.-A. L., Shew, T. M., Abumrad, N. A., & Wolins, N. E. (2013). Perilipin 1 moves between the fat droplet and the endoplasmic reticulum. Adipocyte, 2(2), 80-86.

Sun, Z., Gong, J., Wu, H., Xu, W., Wu, L., Xu, D., . . . Yang, M. (2013). Perilipin1 promotes unilocular lipid droplet formation through the activation of Fsp27 in adipocytes. Nature communications, 4(1), 1594.

Sztalryd, C., & Brasaemle, D. L. (2017). The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1862(10), 1221-1232.

Tansey, J., Sztalryd, C., Hlavin, E., Kimmel, A., & Londos, C. (2004). The central role of perilipin a in lipid metabolism and adipocyte lipolysis. IUBMB life, 56(7), 379-385.

Watt, M. J., & Steinberg, G. R. (2008). Regulation and function of triacylglycerol lipases in cellular metabolism. Biochemical Journal, 414(3), 313-325.

Xu, W., Wu, L., Yu, M., Chen, F.-J., Arshad, M., Xia, X., . . . Xu, D. (2016). Differential Roles of Cell Death-inducing DNA Fragmentation Factor-α-like Effector (CIDE) Proteins in Promoting Lipid Droplet Fusion and Growth in Subpopulations of Hepatocytes*♦. Journal of Biological Chemistry, 291(9), 4282-4293.

Downloads

Published

2024-06-30

Issue

Section

Original Research